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Einband grossNovel Developments in Cryo-EM of Biological Molecules
ISBN/GTIN

Novel Developments in Cryo-EM of Biological Molecules

E-BookPDF0 - No protectionE-Book
552 Seiten
Englisch
Taylor & Franciserschienen am06.10.20231. Auflage
Cryo-EM, as it is currently practiced in many laboratories, is limited to the visualization of molecules that are in thermal equilibrium at the time before freezing. A further limitation is that the existing software does not fully exploit the information that is contained in the images of large ensembles of molecules in thermal equilibrium. This book is a collection of recent articles by the author, reprinted with introductions, and they mainly describe two novel methods in cryo-EM, one computational and the other experimental that requires the use of a microfluidic device. Both methods have the capacity to shed light on the dynamic behavior of biomolecules. Combined, they greatly expand the range of applications of cryo-EM.



The book describes a successful approach in which, based on cryo-EM data, all states visited by the molecule in thermal equilibrium are mapped by manifold embedding-a method of geometric machine learning-and the energy landscape of the molecule is derived. It also discusses methods and biological results of time-resolved cryo-EM, following a reaction in a non-equilibrium system over a short period of time and resulting in the capture of short-lived states that have been inaccessible by standard methods of cryo-EM.mehr
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Produkt

KlappentextCryo-EM, as it is currently practiced in many laboratories, is limited to the visualization of molecules that are in thermal equilibrium at the time before freezing. A further limitation is that the existing software does not fully exploit the information that is contained in the images of large ensembles of molecules in thermal equilibrium. This book is a collection of recent articles by the author, reprinted with introductions, and they mainly describe two novel methods in cryo-EM, one computational and the other experimental that requires the use of a microfluidic device. Both methods have the capacity to shed light on the dynamic behavior of biomolecules. Combined, they greatly expand the range of applications of cryo-EM.



The book describes a successful approach in which, based on cryo-EM data, all states visited by the molecule in thermal equilibrium are mapped by manifold embedding-a method of geometric machine learning-and the energy landscape of the molecule is derived. It also discusses methods and biological results of time-resolved cryo-EM, following a reaction in a non-equilibrium system over a short period of time and resulting in the capture of short-lived states that have been inaccessible by standard methods of cryo-EM.
Details
Weitere ISBN/GTIN9781000989441
ProduktartE-Book
EinbandartE-Book
FormatPDF
Format Hinweis0 - No protection
Erscheinungsjahr2023
Erscheinungsdatum06.10.2023
Auflage1. Auflage
Seiten552 Seiten
SpracheEnglisch
Dateigrösse57569 Kbytes
Artikel-Nr.11845040
Rubriken
Genre9200

Inhalt/Kritik

Inhaltsverzeichnis
Part I: Single-Particle Cryo-EM of Molecules in Thermal Equilibrium

1. Generalized Single-Particle Cryo-EM:. A Historical Perspective

2. Advances in the Field of Single-Particle Cryo-Electron Microscopy Over the Last Decade

3. Single-Particle Reconstruction of Biological Molecules:Story in a Sample (Nobel Lecture)

Part II: Machine Learning Applied to Ensembles of Molecules in Thermal Equilibrium: Resolution in State Space

4. Structural Characterization of mRNA-tRNA Translocation Intermediates

5. Trajectories of the Ribosome as a Brownian Nanomachine

6. Continuous Changes in Structure Mapped by Manifold Embedding of Single-Particle Data in Cryo-EM

7. New Opportunities Created by Single-Particle Cryo-EM: The Mapping of Conformational Space

8. POLARIS: Path of Least Action Analysis on Energy Landscapes

9. Propagation of Conformational Coordinates Across Angular Space in Mapping the Continuum of States from Cryo-EM Data by Manifold Embedding

10. Retrieving Functional Pathways of Biomolecules from Single-Particle Snapshots

11. A Glycan Gate Controls Opening of the SARS-CoV-2 Spike Protein

12. Recovery of Conformational Continuum from Single-Particle Cryo-EM images: Optimization of ManifoldEM Informed by Ground Truth

Part III: Non-Equilibrium Methods: Resolution in Time

13. Structural Dynamics of Ribosome Subunit Association Studied by Mixing-Spraying Time-Resolved Cryo-EM

14. Two Promising Future Developments of Cryo-EM: Capturing Short-Lived States and Mapping a Continuum of States of a Macromolecule

15. Key Intermediates in Ribosome Recycling Visualized by Time-Resolved Cryo-Electron Microscopy

16. A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM

17. Time-Resolved Cryo-Electron Microscopy: Recent Progress

18. Time-Resolved Cryo-Electron Microscopy Using a Microfluidic Chip

19. Late Steps in Bacterial Translation Initiation Visualized Using Time-Resolved Cryo-EM

20. The Structural Basis for Release Factor Activation During Translation Termination Revealed by Time-Resolved Cryogenic Electron Microscopy

21. A Time-Resolved Cryo-EM study of Saccharomyces cerevisiae 80S Ribosome Protein Composition in Response to a Change in Carbon Sourcemehr