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RNA Abundance Analysis

Methods and Protocols
BuchGebunden
233 Seiten
Englisch
Springererschienen am15.08.20202. Aufl.
It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms.mehr
Verfügbare Formate
BuchGebunden
EUR160,49
BuchKartoniert, Paperback
EUR117,69
E-BookPDF1 - PDF WatermarkE-Book
EUR106,99

Produkt

KlappentextIt includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms.
Zusammenfassung
Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts
Details
ISBN/GTIN978-1-0716-0742-8
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2020
Erscheinungsdatum15.08.2020
Auflage2. Aufl.
ReiheMethods in Molecular Biology
Seiten233 Seiten
SpracheEnglisch
Gewicht671 g
IllustrationenXI, 233 p. 12 illus.
Artikel-Nr.16115766

Inhalt/Kritik

Inhaltsverzeichnis
CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in Nicotiana benthamiana.- Strand-Specific RNA-Seq Applied to Malaria Samples.- Laser Microdissection of Cells and Isolation of High-Quality RNA after Cryosectioning.- Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting.- Quantitative Analysis of Plant miRNA Primary Transcripts.- A Revised Adaptation of the Smart-Seq2 Protocol for Single Nematode RNA-Seq.- Analysis of RBP Regulation and Co-Regulation of mRNA 3´ UTR Regions in a Luciferase Reporter System.- Extraction of Small RNAs by Titanium Dioxide Nanofibers.- Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data.- Purification and Analysis of Chloroplast RNAs in Arabidopsis.- In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes.- Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs.- Isolation of Insect Bacteriocytes as a Platform for Transcriptomic Analyses.- Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora crassa and Fusarium oxysporum and Analysis of Small RNAs in Fusarium oxysporum-Infected Plant Root Tissue.- Studying RNA-Protein Interaction Using Riboproteomics.- Small RNA Extraction and Quantification of Isolated Fungal Cells from Plant Tissue by the Sequential Protoplastation.mehr