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Bioreactors in Stem Cell Biology

Methods and Protocols
BuchGebunden
273 Seiten
Englisch
Springererschienen am21.01.20222. Aufl.
Given that the use of bioreactors in cell biology is becoming more commonplace as attempts are made to scale-up production of various types of cells for regenerative medicine and pharmaceutical purposes, this volume provides practical guidance for navigating research projects.mehr
Verfügbare Formate
BuchKartoniert, Paperback
EUR106,99
BuchGebunden
EUR213,99
BuchKartoniert, Paperback
EUR139,09
E-BookPDF1 - PDF WatermarkE-Book
EUR128,39

Produkt

KlappentextGiven that the use of bioreactors in cell biology is becoming more commonplace as attempts are made to scale-up production of various types of cells for regenerative medicine and pharmaceutical purposes, this volume provides practical guidance for navigating research projects.
Zusammenfassung
Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts
Details
ISBN/GTIN978-1-0716-2017-5
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2022
Erscheinungsdatum21.01.2022
Auflage2. Aufl.
ReiheMethods in Molecular Biology
Seiten273 Seiten
SpracheEnglisch
IllustrationenXII, 273 p. 74 illus., 53 illus. in color.
Artikel-Nr.50075658

Inhalt/Kritik

Inhaltsverzeichnis
- Benchtop Bioreactors in Mammalian Cell Culture: Overview and Guidelines. - Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor. - Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter Volume Bioreactors for Cell Culturing. - Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors. - High-Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors. - Integrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture. - Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor. - Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor. - Optimized Method to Improve Cell Activity in 3D Scaffolds Under a Dual Real-Time Dynamic Bioreactor System. - In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor. - Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor. - Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor. - Bioreactor-Based De-epithelialization of Long-Segment Tracheal Grafts. - Production of Extracellular Vesicles Using a CELLine Adherent Bioreactor Flask. - Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors. - Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies: An In Vitro Bioreactor Perfusion Model. - A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3). - Applying Stirred Perfusion to 3D Tissue Equivalents to Mimic the Dynamic In Vivo Microenvironment. - Bioreactor-Based Adherent CellsHarvesting from Microcarriers with 3D Printed Inertial Microfluidics.mehr