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The P2X7 Receptor

Methods and Protocols
BuchGebunden
394 Seiten
Englisch
Springererschienen am02.07.20221st ed. 2022
This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models.mehr
Verfügbare Formate
BuchGebunden
EUR235,39
BuchKartoniert, Paperback
EUR165,84
E-BookPDF1 - PDF WatermarkE-Book
EUR160,49

Produkt

KlappentextThis detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models.
Zusammenfassung
Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts
Details
ISBN/GTIN978-1-0716-2383-1
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2022
Erscheinungsdatum02.07.2022
Auflage1st ed. 2022
ReiheMethods in Molecular Biology
Seiten394 Seiten
SpracheEnglisch
IllustrationenXIV, 394 p. 93 illus., 81 illus. in color.
Artikel-Nr.50432788

Inhalt/Kritik

Inhaltsverzeichnis
How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating.- Agonists, Antagonists, and Modulators of P2X7 Receptors.- Modulation of Cell Energy Metabolism by the P2X7 Receptor.- Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE.- Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4).- Development of Antibody and Nanobody Tools for P2X7.- Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors.- Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations.- Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes.- A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors.- Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents.- Using SymmetricalOrganic Cation Solutions to Study P2X7 Ion Permeation.- Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors.- Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry.- Characterization of P2X7 Receptors in Human Blood Cells.- Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in Multiple Cell Populations Mixed in a Single FACS Tube.- Administration of P2X7 Receptor Blockers in Oncological Experimental Models.- Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease.- Analysis of P2X7-Induced Neuronal Branching.- Studying the Role of P2X7 Receptor in Axonal Growth Using In Utero Electroporation Technique.- Analyzing the Role of the P2X7 Receptor in Epilepsy.mehr