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Bacterial Amyloids

Methods and Protocols
BuchGebunden
337 Seiten
Englisch
Springererschienen am12.08.20221st ed. 2022
This detailed volume introduces different methods to assess the structure and folding of bacterial amyloids and analyze their functions, either in vitro or in vivo.mehr
Verfügbare Formate
BuchGebunden
EUR213,99
BuchKartoniert, Paperback
EUR139,09
E-BookPDF1 - PDF WatermarkE-Book
EUR128,39

Produkt

KlappentextThis detailed volume introduces different methods to assess the structure and folding of bacterial amyloids and analyze their functions, either in vitro or in vivo.
Zusammenfassung
Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts
Details
ISBN/GTIN978-1-0716-2528-6
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2022
Erscheinungsdatum12.08.2022
Auflage1st ed. 2022
ReiheMethods in Molecular Biology
Seiten337 Seiten
SpracheEnglisch
IllustrationenXI, 337 p. 108 illus., 98 illus. in color.
Artikel-Nr.50491206

Inhalt/Kritik

Inhaltsverzeichnis
Study of Amyloid Fibers Using Atomic Force Microscopy.- Cryo-Electron Microscopy to Analyze the Structure of Bacterial Amyloids In Vitro.- Monitoring Molecular Assembly of Biofilms Using Quartz Crystal Microbalance with Dissipation (QCM-D).- Measuring the Size and Spontaneous Fluctuations of Amyloid Aggregates with Fluorescence Correlation Spectroscopy.- Determining the Stoichiometry of Amyloid Oligomers by Single Molecule Photobleaching.- Structural Information on Bacterial Amyloid and Amyloid-DNA Complex Obtained by Small-Angle Neutron/X-Ray Scattering.- Fiber Diffraction and Small-Angle Scattering for Structural Investigation of Bacterial Amyloids.- Supported Lipid Bilayers (SLBs) to Study Amyloid-Lipid Membrane Interactions with Atomic Force Microscopy.- Characterization of Bacterial Amyloids by Nano-Infrared Spectroscopy.- High Throughput Screening of Heterologous Functional Amyloids Using Escherichia coli.- Evaluation of Amyloid Inhibitor Efficiency to Block Bacterial Survival.- Anti-Amyloid Drug Screening Methods Using Bacterial Inclusion Bodies.- Determination of Effects and Mechanisms of Action of Bacterial Amyloids on Antibiotic Resistance.- Design and Testing of Synthetic Catalytic Amyloids Based on the Active Site of Enzymes.- Analysis of Bacterial Amyloid Interaction with Lipidic Membrane by Orientated Circular Dichroism and Infrared Spectroscopies.- Direct, Rapid, and Simple Evaluation of the Expression and Conformation of Beta-Amyloid in Bacterial Cells by FTIR Spectroscopy.- Assessment of Intracellular Amyloid Formation in Fixed and Live Bacteria Using Fluorescence Microscopy.- Identification of Aggregation-Prone and Gatekeeper Residues in Bacterial Amyloids Using Site-Directed Mutagenesis and Flow Cytometry.- Probing DNA-Amyloid Interaction and Gel Formation by Active Magnetic Wire Microrheology.- Probing Amyloids-DNA Interaction with Nanofluidics.- Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo Soft X-Ray Tomography.mehr