Hugendubel.info - Die B2B Online-Buchhandlung 

Merkliste
Die Merkliste ist leer.
Bitte warten - die Druckansicht der Seite wird vorbereitet.
Der Druckdialog öffnet sich, sobald die Seite vollständig geladen wurde.
Sollte die Druckvorschau unvollständig sein, bitte schliessen und "Erneut drucken" wählen.

Chromaffin Cells

Methods and Protocols
BuchKartoniert, Paperback
373 Seiten
Englisch
Springererschienen am09.10.20231st ed. 2023
This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.mehr
Verfügbare Formate
BuchGebunden
EUR160,49
BuchKartoniert, Paperback
EUR117,69

Produkt

KlappentextThis volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
Details
ISBN/GTIN978-1-0716-2673-3
ProduktartBuch
EinbandartKartoniert, Paperback
Verlag
Erscheinungsjahr2023
Erscheinungsdatum09.10.2023
Auflage1st ed. 2023
ReiheMethods in Molecular Biology
Seiten373 Seiten
SpracheEnglisch
IllustrationenXIV, 373 p. 89 illus., 68 illus. in color.
Artikel-Nr.54744904

Inhalt/Kritik

Inhaltsverzeichnis
Rat pheochromocytoma PC12 cells in culture.- Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation.- Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells.- Transmission electron microscopy: a method for studying the adrenochromaffin cells.- Immunogold for protein location in chromaffin cells.- Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy.- Online detection of catecholamine release from the perfused rat adrenal gland.- On-line recording of perifused chromaffin cells.- Recording of chromaffin cell electrical activity in situ in acute adrenal slices.- Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats.- Measurements of calcium in chromaffin cell organelles using targeted aequorins.- Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements.- Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell.- Quantal release analysis of electrochemically active molecules using single-cell amperometry.- Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs.- Vesicle collision protocols for the study of quantum size and exocytotic fraction released.- Patch Amperometry and Intracellular Patch Electrochemistry.- Artificial cells for dissecting exocytosis.- Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells.- Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells.- Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging.- Determinationof catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein.- Quantification of chromogranin A and its fragments in biological fluids.- Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds.mehr