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Phagocytosis and Phagosomes

Methods and Protocols
BuchGebunden
387 Seiten
Englisch
Springererschienen am27.06.20232. Aufl.
This volume explores updated and entirely new experimental approaches used to investigate phagocytosis and phagosome maturation.mehr
Verfügbare Formate
BuchGebunden
EUR246,09
BuchKartoniert, Paperback
EUR176,54
E-BookPDF1 - PDF WatermarkE-Book
EUR234,33

Produkt

KlappentextThis volume explores updated and entirely new experimental approaches used to investigate phagocytosis and phagosome maturation.
Details
ISBN/GTIN978-1-0716-3337-3
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2023
Erscheinungsdatum27.06.2023
Auflage2. Aufl.
ReiheMethods in Molecular Biology
Seiten387 Seiten
SpracheEnglisch
IllustrationenXI, 387 p. 75 illus., 53 illus. in color.
Artikel-Nr.52238895

Inhalt/Kritik

Inhaltsverzeichnis
Bacterial Binding, Phagocytosis, and Killing: Measurements Using Colony Forming Units.- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry.- Quantifying Phagocytosis by Immunofluorescence and Microscopy.- Methods for Quantitative Efferocytosis Assays.- Analysis of Efferocytic Receptor Dynamics and Synapse Formation in a Frustrated Efferocytosis Model.- Super-Resolution Spinning-Disk Confocal Microscopy Using Optical Photon Reassignment (SoRa) to Visualize the Actin Cytoskeleton in Macrophages.- Filamentous Bacteria as Targets to Study Phagocytosis.- The Derivation and Use of HoxB8-Driven Conditionally Immortalized Macrophages.- Quantitative Immunofluorescence to Study Phagosome Maturation and Resolution.- Approaches to Measuring Reductive and Oxidative Events in Phagosomes.- Measuring Phagosomal pH by Fluorescence Microscopy.- Multiplexed Phagosomal Assays for the Detection and Quantification of Bidirectional Exchange Between the Phagolysosomal Lumen and Extracellular Space.- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells.- Measurement of Salmonella enterica Internalization and Vacuole Lysis in Epithelial Cells.- Microscopy-Based Tracking and Quantification Methods to Study Phagosome Resolution.- Isolation of Polystyrene Bead-Induced Phagosomes for Western Blotting.- Biochemically Reconstituted Fusion of Phagosomes with Endosomes and Lysosomes.- An In Vitro System to Analyze Generation and Degradation of Phagosomal Phosphatidylinositol Phosphates.- Examining Phagosomal Pattern Recognition Receptor-Dependent Signaling and Antigen MHC-II Presentation from Phagosomes in Dendritic Cells.- Examining the Kinetics of Phagocytosis-Coupled Inflammasome Activation in Murine Bone Marrow-Derived Dendritic Cells.- Analysis of LC3-Associated Phagocytosis and Antigen Presentation in Macrophages and B Cells.- Visualizing Phagocytic Cargo In Vivo from Engulfment to Resolution in Caenorhabditis elegans.- Assessing the Phagosome Proteome by Quantitative Mass Spectrometry.- Using Ion Substitution and Fluid Indicators to Monitor Macropinosome Dynamics in Live Cells.mehr