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Gene Essentiality

Methods and Protocols
BuchGebunden
248 Seiten
Englisch
Springererschienen am31.01.20152015
This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans.mehr
Verfügbare Formate
BuchKartoniert, Paperback
EUR106,99
BuchGebunden
EUR106,99
E-BookPDF1 - PDF WatermarkE-Book
EUR96,29

Produkt

KlappentextThis volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans.
Zusammenfassung
Includes cutting-edge methods and protocols for the study of microbial gene essentiality

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras
Details
ISBN/GTIN978-1-4939-2397-7
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2015
Erscheinungsdatum31.01.2015
Auflage2015
ReiheMethods in Molecular Biology
Seiten248 Seiten
SpracheEnglisch
Gewicht680 g
IllustrationenXI, 248 p. 42 illus., 15 illus. in color.
Artikel-Nr.15549201
Rubriken
GenreMedizin

Inhalt/Kritik

Inhaltsverzeichnis
Microarray Transposon Tracking for the Mapping of Conditionally Essential Genes in Campylobacter jejuni.- Identifying Essential Streptococcus sanguinis Genes Using Genome-Wide Deletion Mutation.- Defining Essential Genes and Identifying Virulence Factors of Porphyromonas gingivalis by Massively-Parallel Sequencing of Transposon Libraries (Tn-seq).- Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12.- Identification of Genes Essential for Leptospirosis.- Identifying Essential Genes in Mycobacterium tuberculosis by Global Phenotypic Profiling.- Essential Genes in the Infection Model of Pseudomonas aeruginosa-PCR-Based Signature-Tagged Mutagenesis.- Genome-Wide Synthetic Genetic Screening by Transposon Mutagenesis in Candida albicans.- An Integrated Machine-Learning Model to Predict Prokaryotic Essential Genes.- A Statistical Framework for Improving Genomic Annotations of Transposon Mutagenesis (TM) Assigned Essential Genes.- A Proposed Essential Gene Discovery Pipeline: A Campylobacter jejuni Case Study.- Computational Prediction of Essential Metabolic Genes Using Constraint-Based Approaches.- Three Computational Tools for Predicting Bacterial Essential Genes.- Gene Essentiality Analysis Based on DEG 10, an Updated Database of Essential Genes.- A Novel Essential Domain Perspective for Exploring Gene Essentiality.mehr