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Salamanders in Regeneration Research

Methods and Protocols
BuchGebunden
357 Seiten
Englisch
Springererschienen am05.03.20152015
Salamanders in Regeneration Researchmehr
Verfügbare Formate
BuchGebunden
EUR160,49
BuchKartoniert, Paperback
EUR117,69

Produkt

KlappentextSalamanders in Regeneration Research
Zusammenfassung
Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras
Details
ISBN/GTIN978-1-4939-2494-3
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2015
Erscheinungsdatum05.03.2015
Auflage2015
ReiheMethods in Molecular Biology
Seiten357 Seiten
SpracheEnglisch
Gewicht951 g
IllustrationenX, 357 p. 89 illus., 81 illus. in color.
Artikel-Nr.33656165

Inhalt/Kritik

Inhaltsverzeichnis
Variation in Salamanders: An Essay on Genomes, Development, and Evolution.- Maintaining Eastern Newts (Notophthalmus viridescens) for Regeneration Research.- Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl.- Husbandry of Spanish Ribbed Newts (Pleurodeles waltl.- Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies.- Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH.- Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation.- The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration.- High-efficiency Electroporation of the Spinal Cord in Larval Axolotl.- Pseudotyped Retroviruses for Infecting Axolotl.- Thyroxine Induced Metamorphosis in the Axolotl (Ambystoma mexicanum).- Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos.- In vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration.- Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas.- Culture and Transfection of Axolotl Cells.- Isolation and Culture of Neurospheres from the Adult Newt Brain.- Methods for Axolotl Blood Collection, Intravenous Injection and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb.- Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture.-  Long Term Organ Cultures of Newt Hearts.- In vitro Preparation of Newt Inner Ear Sensory Epithelia as a Model for Repair and Regeneration .- Transgenesis in Axolotl (Ambystoma mexicanum).- Generating and Identifying Axolotls with Targeted Mutations using Cas9 RNA-guided Nuclease.- Gene Manipulation for Regenerative Studies using the Iberian Ribbed Newt, Pleurodeles waltl.- Transcriptomics Using Axolotls.- Sal-Site: Research Resources for The Mexican Axolotl.- Data Mining in Newt-omics, the Repository for Omics Data from the Newt.mehr