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Bioluminescence

Methods and Protocols
BuchGebunden
314 Seiten
Englisch
Springererschienen am18.07.20163. Aufl.
This volumepresents detailed laboratory protocols regarding the three major routetechnologies luciferases, efficient opticalprobes, and applications to visualizing molecular events in living subjects.mehr
Verfügbare Formate
BuchGebunden
EUR106,99
BuchKartoniert, Paperback
EUR117,69
BuchGebunden
EUR235,39
BuchGebunden
EUR235,39
BuchKartoniert, Paperback
EUR165,84
BuchKartoniert, Paperback
EUR165,84

Produkt

KlappentextThis volumepresents detailed laboratory protocols regarding the three major routetechnologies luciferases, efficient opticalprobes, and applications to visualizing molecular events in living subjects.
Zusammenfassung
Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras
Details
ISBN/GTIN978-1-4939-3811-7
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2016
Erscheinungsdatum18.07.2016
Auflage3. Aufl.
ReiheMethods in Molecular Biology
Seiten314 Seiten
SpracheEnglisch
Gewicht745 g
IllustrationenXIII, 314 p. 100 illus., 84 illus. in color.
Artikel-Nr.37729790

Inhalt/Kritik

Inhaltsverzeichnis
Label-free Cell Phenotypic Identificationof D-Luciferin as an Agonist for GPR35.- Synthetic BioluminescentCoelenterazine Derivatives.- Molecular Cloning of SecretedLuciferases from Marine Planktonic Copepods.-  How to Fabricate FunctionalArtificial Luciferases for Bioassays.- QuantumYield Determination Based on Photon Number Measurement, Protocols for FireflyBioluminescence Reactions.- Bioluminescent Ligand-ReceptorBinding Assays for Protein or Peptide Hormones.- Bioluminogenic Imaging of AminopeptidaseN in vitro and in vivo.-  Firefly Luciferase-basedSequential Bioluminescence Resonance Energy Transfer (BRET)-FluorescenceResonance Energy Transfer (FRET) Protease Assays.- Monitoring Intracellular pH Changewith a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast andMammalian Cells.- A Protein-Protein InteractionAssay FlimPIA Based on the Functional Complementation of Mutant FireflyLuciferases.- Single-chain Probes for IlluminatingAndrogenicity of Chemicals.- MulticolorImaging of Bifacial Activities of Estrogens.-  CircularPermutation Probes for Illuminating Phosphorylation of Estrogen Receptor.-  Fabricationof Molecular Strain Probes for Illuminating Protein-Protein Interactions.- AnALuc-based Molecular Tension Probe for Sensing Intramolecular Protein-Protein Interactions.- Live Cell Bioluminescence Imagingin Temporal Reaction of G Protein-coupled Receptor for High-throughput Screeningand Analysis.-  Imaging Histone Methylations inLiving Animals.-  Preparation and Assay of Simple Light off Biosensor Based on ImmobilizedBioluminescent Bacteria for General Toxicity Assays.- InVivo Bioluminescent Imaging of ATP-BindingCassette Transporter-Mediated Efflux at the Blood-Brain Barrier.- Theranostic Imaging of CancerGene Therapy.-  Development of a Multicolor BioluminescenceImaging Platform to Simultaneously Investigate Transcription Factor NF-κB Signalingand Apoptosis .- A Multichannel BioluminescenceDetermination platform for Bioassays.- A Bioluminescence Assay Systemfor Imaging Metal Cationic Activities in Urban Aerosols.- LuminescenceImaging: (a) Multi-color Visualization of Ca2+ Dynamics in DifferentCellular Compartments and (b) Video-rate Tumour Detection in a Freely Moving Mouse.-  Photon Counting System for High-sensitivityDetection of Bioluminescence at Optical Fiber End.mehr