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Plant MAP Kinases

Methods and Protocols - Previously published in hardcover
BuchKartoniert, Paperback
266 Seiten
Englisch
Springererschienen am17.09.2016Softcover reprint of the original 1st ed. 2014
Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors.mehr
Verfügbare Formate
BuchGebunden
EUR160,49
BuchKartoniert, Paperback
EUR117,69

Produkt

KlappentextMitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors.
Details
ISBN/GTIN978-1-4939-4589-4
ProduktartBuch
EinbandartKartoniert, Paperback
Verlag
Erscheinungsjahr2016
Erscheinungsdatum17.09.2016
AuflageSoftcover reprint of the original 1st ed. 2014
ReiheMethods in Molecular Biology
Seiten266 Seiten
SpracheEnglisch
Gewicht554 g
IllustrationenXI, 266 p. 33 illus., 17 illus. in color.
Artikel-Nr.40125396

Inhalt/Kritik

Inhaltsverzeichnis
Transient Expression in Arabidopsis Leaf Mesophyll Protoplast System for Cell-based Functional Analysis of MAPK Cascades Signaling.- Quantification of Stress Induced Mitogen-activated Protein Kinase Expressional Dynamic using Quantitative Real-time PCR.- Analysis of MAPK Activities using MAPK-specific Antibodies.- Detection of Protein Phosphorylation and Charge Isoforms using Vertical one-Dimensional Isoelectric Focusing Gels.- Affinity-based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-Tag(TM).- Identification of Constitutively Active AtMPK6 Mutants using a Functional Screen in Saccharomyces Cerevisiae.- Virus-induced Gene Silencing in Plant MAPK Research.- RNA Interference of Plant MAPK Cascades for Functional Studies.- Immunofluorescent Localization of MAPKs and Colocalization with Microtubules in Arabidopsis Seedling Whole Mount Probes.- Immunofluorescent Localization of MAPKs in Steedman´s Wax Sections.- Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization Studies.- Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases.- Determination of Phosphorylation Sites in Microtubule Associated Protein MAP65-1.- In vivo Phosphorylation of WRKY Transcription Factor by MAPK.- Rapid Mutagenesis-based Analysis of Phosphorylation Sites in Mitogen-activated Protein Kinase Substrates.- Yeast Two-hybrid System for Dissecting the Rice MAPK Interactome.- Experimental and Analytical Approaches to Characterize Plant Kinases using Protein Microarrays.mehr