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Chloroplast Research in Arabidopsis

Methods and Protocols, Volume I - Previously published in hardcover
BuchKartoniert, Paperback
374 Seiten
Englisch
Springererschienen am23.08.2016Softcover reprint of the original 1st ed. 2011
In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system.mehr
Verfügbare Formate
BuchGebunden
EUR106,99
BuchKartoniert, Paperback
EUR155,14

Produkt

KlappentextIn Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system.
ZusammenfassungResearch into chloroplast biology has been hampered by heterogeneous methodologies and subject species. Now Arabidopsis has been adopted as the model research organism, this first part of a two-volume set covers issues such as genetics and cytology.
Details
ISBN/GTIN978-1-4939-5850-4
ProduktartBuch
EinbandartKartoniert, Paperback
Verlag
Erscheinungsjahr2016
Erscheinungsdatum23.08.2016
AuflageSoftcover reprint of the original 1st ed. 2011
ReiheMethods in Molecular Biology
Seiten374 Seiten
SpracheEnglisch
Gewicht728 g
IllustrationenX, 374 p.
Artikel-Nr.43959036

Inhalt/Kritik

Inhaltsverzeichnis
Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis.- Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy.- Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins.- Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts.- Visualisation of Stromules on Arabidopsis Plastids.- Analysis of Chloroplast Movement and Relocation in Arabidopsis.- Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots.- Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy.- Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method.- Isolation, Quantification, and Analysis of Chloroplast DNA.- Measurement of Transcription Rates in Arabidopsis Chloroplasts.- Studying the Structure and Processing of Chloroplast Transcripts.- In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts.- Studying Translation in Arabidopsis Chloroplasts.- Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts.- In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins.- Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays.- Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions.- Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays.- Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction.- Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases.mehr