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Plant Receptor Kinases

Methods and Protocols
BuchGebunden
196 Seiten
Englisch
Springererschienen am01.06.20171st ed. 2017
Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways.mehr
Verfügbare Formate
BuchGebunden
EUR149,79
BuchKartoniert, Paperback
EUR139,09
E-BookPDF1 - PDF WatermarkE-Book
EUR128,39

Produkt

KlappentextChapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways.
Zusammenfassung
Includes cutting-edge methods and protocols

Provides step-by-step detail essential fro reproducible methods

Contains key notes and implementation advice from the experts
Details
ISBN/GTIN978-1-4939-7062-9
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2017
Erscheinungsdatum01.06.2017
Auflage1st ed. 2017
ReiheMethods in Molecular Biology
Seiten196 Seiten
SpracheEnglisch
Gewicht594 g
IllustrationenXII, 196 p. 41 illus., 18 illus. in color.
Artikel-Nr.42527229

Inhalt/Kritik

Inhaltsverzeichnis
Expression of Plant Receptor Kinases in E. coli.- Expression of Plant Receptor Kinases in Yeast.- Expression of Plant Receptor Kinases in Tobacco BY-2 Cells.- Cell-free Synthesis of Plant Receptor Kinases.- Purification of Plant Receptor Kinases from Plant Plasma Membranes.- Photoaffinity Labeling of Plant Receptor Kinases.- Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases.- Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis.- In silico Prediction of Ligand Binding Sites of Plant Receptor Kinases using Conservation Mapping.- Co-immunoprecipitation of Plant Receptor Kinases.- Autophosphorylation Assays using Plant Receptor Kinases Synthesized in Cell-free Systems.- Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners.- In vitro Assessment of Guanylyl Cyclase Activity ofPlant Receptor Kinases.- BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases.- Pull Down Assay to Characterize Ca2+/Calmodulin Binding to Plant Receptor Kinases.- The use of FRET/FLIM to Study Proteins Interacting with Plant Receptor Kinases.- Detection and Analyses of Endocytosis of Plant Receptor Kinases.mehr