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Membrane Protein Structure and Function Characterization

Methods and Protocols
BuchGebunden
419 Seiten
Englisch
Springererschienen am30.07.20171st ed. 2017
In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane.mehr
Verfügbare Formate
BuchKartoniert, Paperback
EUR149,79
BuchGebunden
EUR149,79

Produkt

KlappentextIn this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane.
Zusammenfassung
Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras

Includes supplementary material: sn.pub/extras
Details
ISBN/GTIN978-1-4939-7149-7
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2017
Erscheinungsdatum30.07.2017
Auflage1st ed. 2017
ReiheMethods in Molecular Biology
Seiten419 Seiten
SpracheEnglisch
Gewicht989 g
IllustrationenXII, 419 p. 42 illus., 13 illus. in color.
Artikel-Nr.42665597

Inhalt/Kritik

Inhaltsverzeichnis
Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2.- Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex.- Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris protoplasts.- Cell-free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-synthase subunits.- Wheat Germ Cell Free Overexpression for the Production of Membrane Proteins.- Methyl-specific Isotope Labelling Strategies for NMR Studies of Membrane Proteins.- Labeling of Membrane Complexes for Electron Microscopy.- Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins Case Study of The Mouse 5-HT Receptor.- Characterization of New Detergent and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization.- Secondary Structure Determination by Means of ATR-FTIR Spectroscopy.- Native Mass Spectroscopy forthe Characterization of Structure and Interactions of Membrane Proteins.- Mass Spectroscopy of Mitochondrial Membrane Proteins Complexes.- Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+NQR from V cholerae in the Presence of Lipids.- Reconstitution of Membrane Protein in Liposomes.- Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes.- The CRACAM Robot: Two Dimensional Crystallization of Membrane Protein.- Reconstitution of Membrane Proteins into Nanodiscs for Single Particle Electron Microscopy.- Solid-state NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO.- Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.- Simulation of Ligand Binding To Membrane Proteins.- Molecular modelling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO Molecular Dynamic of Membrane Protein in Different Environments.mehr