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Digital PCR

Methods and Protocols - Previously published in hardcover
BuchKartoniert, Paperback
557 Seiten
Englisch
Springererschienen am10.12.2019Softcover reprint of the original 1st ed. 2018
This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens.mehr
Verfügbare Formate
BuchGebunden
EUR320,99
BuchKartoniert, Paperback
EUR234,33

Produkt

KlappentextThis volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens.
Details
ISBN/GTIN978-1-4939-9282-9
ProduktartBuch
EinbandartKartoniert, Paperback
Verlag
Erscheinungsjahr2019
Erscheinungsdatum10.12.2019
AuflageSoftcover reprint of the original 1st ed. 2018
ReiheMethods in Molecular Biology
Seiten557 Seiten
SpracheEnglisch
Gewicht1070 g
IllustrationenXV, 557 p. 141 illus., 128 illus. in color.
Artikel-Nr.46678962
Rubriken
GenreMedizin

Inhalt/Kritik

Inhaltsverzeichnis
Entering the Pantheon of 21st Century Molecular Biology Tools: A Perspective on Digital PCR.- Basic Concepts and Validation of Digital PCR Measurements.- Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies, What Does it Mean?.- Control Materials and Digital PCR Methods for Evaluation of Circulating Cell Free DNA Extractions from Plasma.- Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events.- Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples.- Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR.- Testing of General and Human-Associated Fecal Contamination in Waters.- Analyzing Copy Number Variation with Droplet Digital PCR.- Assessing HER2 Amplification in Plasma cfDNA.- Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues using ddPCR: analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants.- Monitoring of Response and Resistance in Plasma of EGFR-Mutant Lung Cancer using Droplet Digital PCR.- Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients.- Droplet Digitial PCR for Miniaml Residual Disease Detection in Mature Lymphoproliferative Disorders.- Quantitation of JAK2 V617F Allele Burden by using the QuantStudio(TM) 3D Digitial PCR System.- Novel Multiplexing Strategies for Quantification of Rare Alleles using ddPCR.- Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNA.- Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping.- A Universal Droplet Digital PCR Approach for Monitoring of Graft Health after Transplantation using a Pre-Selected SNP Set.- Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci using Droplet Digital PCR.- DNA Methylation Analysis Using Droplet Digital PCR.- Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts using Droplet Digital PCR.- Using Droplet Digital PCR to Analyze Allele-Specific RNA Expression.- Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCR(TM) Assays.- Quantification of Circulating MicroRNAs by Droplet Digital PCR using EvaGreen Probeless Chemistry.- Droplet Digital PCR for Absolute Quantification of Extracellular MicroRNAs in Plasma and Serum: Quantification of the Cancer Biomarker has-miR-141.- Droplet Digital(TM) PCR Next-Generation Sequencing Library QC Assay.- Phasing DNA Markers using Digital PCR.- ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity.- Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method.mehr
Kritik
"The editors of the volume have succeeded in providing specialists in different sectors with a wide range of technology applications that can be adapted to other platforms or can provide the basis for creating new ones." (Paolo Catarsi, European Journal of Histochemistry, Vol. 63, 2019)mehr