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Stem Cells and Aging

Methods and Protocols
BuchKartoniert, Paperback
354 Seiten
Englisch
Springererschienen am05.09.20202. Aufl.
This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging.mehr
Verfügbare Formate
BuchKartoniert, Paperback
EUR128,39
BuchGebunden
EUR181,89
BuchKartoniert, Paperback
EUR128,39
BuchGebunden
EUR106,99

Produkt

KlappentextThis fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging.
Zusammenfassung
Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts
Details
ISBN/GTIN978-1-4939-9715-2
ProduktartBuch
EinbandartKartoniert, Paperback
Verlag
Erscheinungsjahr2020
Erscheinungsdatum05.09.2020
Auflage2. Aufl.
ReiheMethods in Molecular Biology
Seiten354 Seiten
SpracheEnglisch
IllustrationenXII, 354 p. 67 illus., 64 illus. in color.
Artikel-Nr.49019100

Inhalt/Kritik

Inhaltsverzeichnis
Assessing Muscle Stem Cell Clonal Complexity during Aging.- Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.- Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle.- Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue.- Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures.- Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells.- Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis.- Complete Assessment of Multilineage Differentiation Potential of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells.- Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis.- 3D-Embedded Cell Cultures to Study Tendon Biology.- Targeted, Amplicon-Based,Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis.- Column Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants.- Histological Assessment of Cre/loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice.- Infrared Spectroscopy and Imagıng in Stem Cells and Agıng Research.- Use of U-STELA for Accurate Measurement of Extremely Short-Telomeres.- Surface-Antigen-Based Identification of In Vitro Expanded Skeletal-Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry.- Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry.- Methods for Detection of Autophagy in Mammalian Cells.- Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells.- Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer.- Generation of Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD).- Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury.- 3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol.- Isolation, Expansion, and Characterization of Wharton´s Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments.- CRISPR Base Editing in Induced Pluripotent Stem Cells.mehr