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Fundamental Approaches to Screen Abnormalities in Drosophila

BuchGebunden
265 Seiten
Englisch
Springererschienen am18.01.20201st ed. 2020
This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila.mehr
Verfügbare Formate
BuchGebunden
EUR160,49
BuchKartoniert, Paperback
EUR117,69

Produkt

KlappentextThis volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila.
Details
ISBN/GTIN978-1-4939-9755-8
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2020
Erscheinungsdatum18.01.2020
Auflage1st ed. 2020
ReiheSpringer Protocols Handbooks
Seiten265 Seiten
SpracheEnglisch
Gewicht728 g
IllustrationenVII, 265 p. 116 illus., 111 illus. in color.
Artikel-Nr.46757020

Inhalt/Kritik

Inhaltsverzeichnis
Chapter 1. Preparation of normal food and modified food for Drosophila.- Chapter 2. Protocol for dissection of various tissues (gut, imaginal disc, fat body, ovary, testes) in larva and adult fly.- Chapter 3. Biochemical estimation of various metabolites (protein, lipid, carbohydrate, trehalose, cholesterol).- Chapter 4. Detection of fat within the Drosophila body.- Chapter 5. Estimation of reactive oxygen species (ROS) production  in Drosophila cells.- Chapter 6. Methods to check the metabolic activity of fly.- Chapter 7. Detection of DNA damage in larva and adult stage of Drosophila by Fluorescent microscopy.- Chapter 8. Imaging of larva/adult gut damage using Bright field microscopy.- Chapter 9. A comet assay to detect severity of DNA damage.- Chapter 10. Checking the abnormality in hemolymph by Giemsa stain.- Chapter 11. Analysis of channel proteins.- Chapter 12. Various behavioral assays in larva and adult Drosophila.- Chapter 13. Detection of various metals within the tissue by Energy dispersive X-ray (EDX) through SEM and FTIR.- Chapter 14. Imaging of various sections of fly through Scanning Electron Microscopy (SEM).- Chapter 15. Preparation of thin sections and fixation of various tissues of Drosophila for examination by transmission electron microscopy (TEM).- Chapter 16. Flow cytometric analysis of Drosophila cells.mehr

Schlagworte

Autor

Dr. Monalisa Mishra is an Assistant Professor at the Department of Life Sciences, National Institute of Technology (NIT) in Rourkela, India. She completed her Ph.D. at the University of Bremen, Germany, prior to postdoctoral studies at the School of Medicine, University of San Diego, California, USA; Department of Biological Sciences, Indiana University, Bloomington, USA; and Max Plank Institute of Cell Biology and Genetics, Dresden, Germany.

Further, she previously served as an Assistant Professor at the Department of Biological Sciences, BITS in Pilani, India from 2012-2014. Currently, her lab at the NIT Rourkela is investigating various factors that can affect the development of Drosophila's sensory organs. The lab's findings have been published in many prominent international journals.
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