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2D PAGE: Sample Preparation and Fractionation

Volume 2
BuchGebunden
340 Seiten
Englisch
Springererschienen am19.03.2008
This book sets forth principles and recent developments of two-dimensional electrophoresis in expression proteomics. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time.mehr
Verfügbare Formate
BuchGebunden
EUR106,99
BuchKartoniert, Paperback
EUR149,79
E-BookPDF1 - PDF WatermarkE-Book
EUR96,29

Produkt

KlappentextThis book sets forth principles and recent developments of two-dimensional electrophoresis in expression proteomics. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time.
Details
ISBN/GTIN978-1-60327-209-4
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2008
Erscheinungsdatum19.03.2008
ReiheMethods in Molecular Biology
Seiten340 Seiten
SpracheEnglisch
IllustrationenXIII, 340 p. 83 illus., 3 illus. in color.
Artikel-Nr.10817263

Inhalt/Kritik

Inhaltsverzeichnis
Application of Fluorescence Dye Saturation Labeling for Differential Proteome Analysis of 1,000 Microdissected Cells from Pancreatic Ductal Adenocarcinoma Precursor Lesions.- Albumin and Immunoglobulin Depletion of Human Plasma.- Multi-Component Immunoaffinity Subtraction and Reversed-Phase Chromatography of Human Serum.- Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies.- Proteomics of Cerebrospinal Fluid: Methods for Sample Processing.- Sample Preparation of Bronchoalveolar Lavage Fluid.- Preparation of Nasal Secretions for Proteome Analysis.- Preparation of Urine Samples for Proteomic Analysis.- Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis.- Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells.- Sample Preparation for Mass Spectrometry Analysis of Formalin-Fixed Paraffin-Embedded Tissue.- Metalloproteomics in the Molecular Study of Cell Physiology and Disease.- Protein Extraction from Green Plant Tissue.- The Terminator: A Device for High-Throughput Extraction of Plant Material.- Isolation of Mitochondria from Plant Cell Culture.- Isolation and Preparation of Chloroplasts from Arabidopsis thaliana Plants.- Isolation of Plant Cell Wall Proteins.- Isolation and Fractionation of the Endoplasmic Reticulum from Castor Bean (Ricinus communis) Endosperm for Proteomic Analyses.- Cell Wall Fractionation for Yeast and Fungal Proteomics.- Collection of Proteins Secreted from Yeast Protoplasts in Active Cell Wall Regeneration.- Sample Preparation Procedure for Cellular Fungi.- Isolation and Enrichment of Secreted Proteins from Filamentous Fungi.- Isolation and Solubilization of Cellular Membrane Proteins from Bacteria.- Isolation and Solubilization of Gram-Positive Bacterial Cell Wall-Associated Proteins.- Cell Fractionation of Parasitic Protozoa.mehr

Autor

Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. 2-DE, with its unique capacity to resolve thousands of proteins in a single run, remains a fundamental research tool for nearly all protein-related scientific projects. Step-by-step instructions alongside detailed illustrations enable biochemists, molecular and cellular biologists, and protein scientists to quickly learn how to perform the methods. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.