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Light Microscopy

Methods and Protocols
BuchGebunden
244 Seiten
Englisch
Springererschienen am15.12.2010
This book explores the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques. Each chapter offers step-by-step methods, lists of necessary materials, and tips on troubleshooting and avoiding known pitfalls.mehr
Verfügbare Formate
BuchGebunden
EUR149,79
BuchKartoniert, Paperback
EUR106,99

Produkt

KlappentextThis book explores the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques. Each chapter offers step-by-step methods, lists of necessary materials, and tips on troubleshooting and avoiding known pitfalls.
Zusammenfassung
Readily reproducible protocols for optimal histological observations under bright-field microscopy

Provides full description of plastic resin embedding methods for plant and animal research

Presents advanced methods to study leukocyte biology and inflammatory responses

Imaging techniques applied to cytogenetic, cancer, neurobiology, ion mobilization, and aquatic ecology studies

Includes immunocytochemistry methods and a comprehensive overview of fluorescence microscopy

Includes supplementary material: sn.pub/extras
Details
ISBN/GTIN978-1-60761-949-9
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2010
Erscheinungsdatum15.12.2010
ReiheMethods in Molecular Biology
Seiten244 Seiten
SpracheEnglisch
Gewicht634 g
IllustrationenX, 244 p.
Artikel-Nr.11418777

Inhalt/Kritik

Inhaltsverzeichnis
Glycol methacrylate embedding for improved morphological, morphometrical and immunohistochemical investigations under light microscopy: testes as a model.- Histological processing of teeth and periodontal tissues for light microscopy analysis.- Large plant samples: how to process for 2-hydroxyethyl methacrylate embedding?.- Image cytometry: nuclear and chromosomal DNA quantification.- Histological approaches to study tissue parasitism during Trypanosoma cruzi infection.- Intravital microscopy to study leukocyte recruitment in vivo.- Introduction to fluorescence microscopy.- Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals.- Imaging lipid bodies within leukocytes with different light microscopy techniques.- EicosaCell - An immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites.- Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer.- Imaging calcium sparks in cardiac myocytes.- Light microscopy in aquatic ecology: methods for plankton communities studies.- Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedding samples.mehr
Kritik
From the reviews:

"The book is superbly illustrated with the great majority of the figures in colours and is certainly a must not only for beginners ... but I am pretty sure that even the practised colleagues in doing every day use of microscopic methods will find very useful tricks ... . thanks to this well planned and clearly written book we are stimulated to imagine new applications." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)mehr