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Strain Engineering

Methods and Protocols
BuchGebunden
480 Seiten
Englisch
Springererschienen am15.07.2011
Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications.mehr
Verfügbare Formate
BuchGebunden
EUR160,49
BuchKartoniert, Paperback
EUR117,69
E-BookPDF1 - PDF WatermarkE-Book
EUR106,99

Produkt

KlappentextClassical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications.
ZusammenfassungThis book presents powerful new genetic engineering-based strain engineering methods for rational modification of a variety of model organisms. It contains key notes and implementation advice from the experts.
Details
ISBN/GTIN978-1-61779-196-3
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2011
Erscheinungsdatum15.07.2011
Seiten480 Seiten
SpracheEnglisch
Gewicht1050 g
IllustrationenXI, 480 p. 83 illus., 2 illus. in color.
Artikel-Nr.10334352
Rubriken
GenreMedizin

Inhalt/Kritik

Inhaltsverzeichnis
Bacterial Genome Reengineering.- Targeted Chromosomal Gene Knockout using PCR Fragments.- Scarless Chromosomal Gene Knockout Methods.- Random Chromosomal Gene Disruption In vivo using Transposomes.- Genome Engineering using Targeted Oligonucleotide Libraries and Functional Selection.- Microarray-based Genetic Footprinting Strategy to Identify Strain Improvement Genes after Competitive Selection of Transposon Libraries.- Optimization of Synthetic Operons using Libraries of Post-Transciptional Regulatory Elements.- Marker Free Chromosomal Expression of Foreign and Native Genes in E. coli.- Array-based Synthetic Genetic Screens to Map Bacterial Pathways and Functional Networks in Escherichia coli.- Assembling New E. coli Strains by Transduction using Phage P1.- Yeast Bioinformatics and Strain Engineering Resources.- Delete and Repeat: A Comprehensive Toolkit for Sequential Gene Knock-out in the Budding Yeast Saccharomyces cerevisiae.- Genome-wide Transposon Mutagenesis in Saccharomyces cerevisiae and Candida albicans.- Signature-tagged Mutagenesis to Characterize Genes Through Competitive Selection of Barcoded Genome Libraries.- Global Strain Engineering by Mutant Transcription Factors.- Genomic Promoter Replacement Cassettes to Alter Gene Expression in the Yeast Saccharomyces cerevisiae.- Microbial Genome Analysis and Comparisons: Web-based Protocols and Resources.- Plasmid Artificial Modification: A Novel Method for Efficient DNA Transfer into Bacteria.- Broad-host-range Plasmid Vectors for Gene Expression in Bacteria.- Simple Method for Introducing Marker-free Deletions in the Bacillus subtilis Genome.- Transposon-mediated Random Mutagenesis of Bacillus subtilis.- Integrative Food Grade Expression System for Lactic Acid Bacteria.- ClosTron-mediated Engineering of Clostridium.- High Throughput Transposon Mutagenesis of Corynebacterium glutamicum.- Mini-Mu Transposon Mutagenesis of Ethanologenic Zymomonas mobilis.- Engineering Thermoacidophilic Archaea using Linear DNA Recombination.- Targeted Gene Disruption in Koji Mold Aspergillus oryzae.- Selectable and Inheritable Gene Silencing through RNA Interference in the Unicellular Alga Chlamydomonas reinhardtii.mehr