Hugendubel.info - Die B2B Online-Buchhandlung 

Merkliste
Die Merkliste ist leer.
Bitte warten - die Druckansicht der Seite wird vorbereitet.
Der Druckdialog öffnet sich, sobald die Seite vollständig geladen wurde.
Sollte die Druckvorschau unvollständig sein, bitte schliessen und "Erneut drucken" wählen.
BuchGebunden
607 Seiten
Englisch
Springererschienen am02.12.20112nd ed.
New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength.mehr
Verfügbare Formate
BuchGebunden
EUR160,49
BuchKartoniert, Paperback
EUR117,69

Produkt

KlappentextNew pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength.
ZusammenfassungThe past decade has seen major advances in our understanding of bone biology. This revised and updated second edition includes the step-by-step detail essential for reproducible results and features expert commentary on numerous study techniques.
Details
ISBN/GTIN978-1-61779-414-8
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2011
Erscheinungsdatum02.12.2011
Auflage2nd ed.
ReiheMethods in Molecular Biology
Seiten607 Seiten
SpracheEnglisch
Gewicht1348 g
IllustrationenXIV, 607 p.
Artikel-Nr.11504085

Inhalt/Kritik

Inhaltsverzeichnis
Primary Human Osteoblast Cultures.- Osteoblast Isolation from Murine Calvaria and Long Bones.- Rat Osteoblast Cultures.- Isolation of primary avian Osteocytes.- Isolation of Mouse Osteocytes using Cell Fractionation for Gene Expression Analysis.- Studying Osteocyte Function using the Cell Lines MLO-Y4 and MLO-A5.- Isolation, Differentiation and Characterisation of Human Bone Marrow Stem Cells in vitro and in vivo.- Rodent Osteoclast Cultures.- Isolation and Culture of Primary Chicken Osteoclasts.- ISOLATION AND PURIFICATION OF RABBIT OSTEOCLASTS.- Generation of Human Osteoclasts from Peripheral Blood.- Osteoclast Formation in Mouse Co-Cultures.- Rankl- Mediated Osteoclast Formation from Murine RAW 264.7 Cells .- Transfection of Osteoclasts and Osteoclast Precursors.- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation.- Analysis of Transcriptional Regulation in Bone Cells.- Extraction of Nucleic Acids from Bone.- Analysis of Gene Expression in Bone by Quantitative RT/PCR .- Histomorphometry in Rodents.- Studying Gene Expression in Bone by in situ Hybridization.- Immunostaining of Skeletal Tissue.- Techniques for the Study of Apoptosis in Bone.- Transmission Electron Microscopy of Bone.- Scanning Electron Microscopy of Bone.- Fluorescence Imaging of Osteoclasts using Confocal Microscopy.- Live Imaging of Bone Cell and Organ Cultures.- Analysis of Bone Architecture in Rodents using Micro Computed Tomography.- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents.- Quantitative X-ray Imaging of Rodent Bone by Faxitron.- Bioluminescence Imaging of Bone Metastasis in Rodent.- Fourier Transform Infrared Imaging of Bone.- Raman Microscopy of Bone.- The Calvarial Injection Assay.- Ovariectomy/Orchidectomy in Rodents.- Mechanical Properties of Bone ex vivo.- Mechanical Stimulation of Bone Cells using Fluid Flow.- using CELL and organ culture models TO analyse responses OF bone cellS to mechanical STIMULATION .In vivo Mechanical Loading.mehr
Kritik
From the reviews of the second edition:
"This volume presents detailed modern experimental methods to study bone biology. For bone researchers." (Pediatric Endocrinology Reviews (PER), Vol. 9 (3), March, 2012)mehr