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Bioluminescent Imaging

Methods and Protocols
BuchGebunden
276 Seiten
Englisch
Springererschienen am29.10.20132014
Yet another contribution to the highly successful Methods in Molecular Biology series, this guide to the techniques of bioluminescent imaging has stepwise protocols on cutting-edge methods widely used in research and medicine to track biological processes.mehr
Verfügbare Formate
BuchKartoniert, Paperback
EUR112,34
BuchGebunden
EUR106,99
E-BookPDF1 - PDF WatermarkE-Book
EUR96,29

Produkt

KlappentextYet another contribution to the highly successful Methods in Molecular Biology series, this guide to the techniques of bioluminescent imaging has stepwise protocols on cutting-edge methods widely used in research and medicine to track biological processes.
Details
ISBN/GTIN978-1-62703-717-4
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2013
Erscheinungsdatum29.10.2013
Auflage2014
ReiheMethods in Molecular Biology
Seiten276 Seiten
SpracheEnglisch
Gewicht672 g
IllustrationenXI, 276 p. 50 illus., 39 illus. in color.
Artikel-Nr.29465436
Rubriken
GenreMedizin

Inhalt/Kritik

Inhaltsverzeichnis
Bioluminescence Imaging: Basics and Practical Limitations.- Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells.- Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter.- Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture.- Split Gaussia Luciferase for Imaging Ligand-Receptor Binding.- Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell.- Bioluminescence Reporter Gene-Based Detection of MicroRNAs.- Monitoring of Transcriptional Dynamics of HIF and NFκB Activities.- Real-Time Bioluminescent Tracking of Cellular Population Dynamics.- Fabrication of Bioluminescent Capsules and Live-Cell Imaging.- The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice.- Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis.- Bioluminescence Imaging of Fungal Biofilm Development in Live Animals.- Bioluminescent Imaging of Bacteria During Mouse Infection.- Cell-Based Bioluminescence Screening Assays.- Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice.- Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase.- Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay.- Non-Invasive In Vivo Monitoring of Extracellular Vesicles.- In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL).mehr
Kritik
From the reviews:
"The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. ... The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields." (Omer Iqbal, Doody's Book Reviews, January, 2014)mehr