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Gigaseal Formation in Patch Clamping

With Applications of Nanotechnology
BuchKartoniert, Paperback
94 Seiten
Englisch
Springererschienen am07.08.20132014
This book presents an investigation of gigaseal formation using micro/nanotechnology. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry.mehr
Verfügbare Formate
BuchKartoniert, Paperback
EUR53,49
E-BookPDF1 - PDF WatermarkE-Book
EUR53,49

Produkt

KlappentextThis book presents an investigation of gigaseal formation using micro/nanotechnology. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry.
Zusammenfassung
Explains the mechanisms of gigaseal formation to the latest level of discoveries

Provides practical techniques, which enable frequent formation of high resistance seals

Shares authors´ recent practice in acquiring gigaseal formation using micropipettes

Includes supplementary material: sn.pub/extras
Details
ISBN/GTIN978-3-642-39127-9
ProduktartBuch
EinbandartKartoniert, Paperback
Verlag
Erscheinungsjahr2013
Erscheinungsdatum07.08.2013
Auflage2014
Seiten94 Seiten
SpracheEnglisch
Gewicht178 g
IllustrationenXI, 94 p. 81 illus., 65 illus. in color.
Artikel-Nr.29034093

Inhalt/Kritik

Inhaltsverzeichnis
Chapter 1 Introduction.- Chapter 2 Development of patch clamping.- Chapter 3 Gigaseal formation.- Chapter 4 Effect of roughness on gigaseal formation.- Chapter 5 Effect of hydrophilicIty on gigaseal formation.- Chapter 6 Effect of tip size on gigaseal formation.- Chapter 7 Study of glass micropipettes from tip formation to characterization.- References.mehr
Kritik
"This is a very good book on patch clamp protocol with a focus on the engineering and physics of pipettes. A description on tuning for bore diameter and various approaches to the culture dishes is detailed. ... This is a reliable resource for use in the physiology labs. Physiologists, students, postdocs, & senior investigators will find this very helpful." (Joseph J. Grenier, Amazon.com, September, 2015)mehr