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BuchKartoniert, Paperback
298 Seiten
Englisch
Springererschienen am03.10.2013Softcover reprint of the original 1st ed. 1974
In addition to the up-to-date presentation of con­ ventional methods of cell culture and techniques for the preparation and identification of human chromosomes, this text covers the various tech­ niques of producing banding patterns and applying them in chromo­ some identification.mehr
Verfügbare Formate
BuchKartoniert, Paperback
EUR53,49
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Produkt

KlappentextIn addition to the up-to-date presentation of con­ ventional methods of cell culture and techniques for the preparation and identification of human chromosomes, this text covers the various tech­ niques of producing banding patterns and applying them in chromo­ some identification.
Details
ISBN/GTIN978-3-642-65789-4
ProduktartBuch
EinbandartKartoniert, Paperback
Verlag
Erscheinungsjahr2013
Erscheinungsdatum03.10.2013
AuflageSoftcover reprint of the original 1st ed. 1974
Seiten298 Seiten
SpracheEnglisch
IllustrationenXV, 298 p. 66 illus.
Artikel-Nr.29949669

Inhalt/Kritik

Inhaltsverzeichnis
I Cell Cultures from Blood and Bone Marrow.- 1. Introduction.- 2. Bone Marrow.- 3. Culture of Peripheral Blood Cells.- 4. Phytohemagglutinin: Properties and Mode of Action.- Appendix: Laboratory Procedures.- References.- II Cell Cultures from Tissue Expiants.- 1. Introduction.- 2. Areas of Application.- 3. Material.- 4. Introduction to Methods.- 5. Techniques for Collecting Tissue.- 6. Setting up Tissue Expiants and Culturing Cells.- 7. Harvest of Cultures for Chromosome Preparation.- 8. Long Term Preservation of Tissue Culture Cells.- Appendix I: Summary of Some of the Methods.- Appendix II: Catalog of Some Reagents, Media and Culture Vessels.- References.- III Culture and Preparation of Cells from Amniotic Fluid.- 1. Introduction.- 2. Collecting and Transportation of Amniotic Fluid.- 3. X- and Y-Chromatin.- 4. Cell Culture.- Appendix: Protocol for Amniotic Fluid Cultures.- References.- IV Preparation of Metaphase Chromosomes.- 1. Introduction.- 2. Preparation of Cell Suspensions.- 3. Direct Preparation from Monolayer Cultures.- 4. Direct Preparation of Embryonic Tissue.- 5. Staining.- Appendix: Laboratory Guide.- References.- V Fluorescence Microscopy of Chromosomes and Interphase Nuclei.- 1. Introduction.- 2. Fluorescent Dyes for Chromosomes and Cell Nucle.- 3. Other Fluorochromes.- 4. Technique of Fluorescence Microscopy.- 5. Analysis of Preparations.- Appendix 1: Staining with Quinacrine-Dihydrochloride and Quinacrine-Mustard.- Appendix 2: Suppliers of Quinacrine Stains.- Appendix 3: Phosphate Buffer Solution (Sörensen).- References.- VI Banding Patterns in Human Chromosomes Visualized by Giemsa Staining after Various Pretreatments.- 1. Introduction.- 2. General Comments on the Different Methods.- 3. Methods for Demonstrating the Centromeric Heterochromatin.- 4.Methods for Demonstrating Banding Patterns over the Entire Chromosome.- Appendix: Laboratory Procedures.- References.- VII Autoradiography of Human Chromosomes with 3H-Thymidine.- 1. Introduction.- 2. Theoretical Basis.- 3. Techniques.- Appendix: Summary of the Procedure.- References.- VIII The Human Karyotype Analysis of Chromosomes in Mitosis and Evaluation of Cytogenetic Data.- 1. Introduction.- 2. Chromosomes at Normal Metaphase.- 3. The Standard Karyotype.- 4. Identification of Individual Chromosomes by Special Methods.- 5. Variability of the Karyotype.- 6. Evaluation of Cytogenetic Data.- 7. Nomenclature.- Appendix: Tables.- References.- IX Analysis of Interphase Nuclei.- 1. Introduction.- 2. Y-Chromatin.- 3. X-Chromatin.- 4. Techniques for Preparing Interphase Nuclei.- 5. Staining.- 6. Evaluation of the Preparations.- Appendix: Laboratory Procedures.- References.- X The Diagnosis of X-Chromatin by the Leukocyte Test.- 1. Introduction.- 2. Methods.- 3. Evaluation.- References.- XI Chromosomes in Meiosis.- 1. Introduction.- 2. Review of the Meiotic Process in Man.- 3. Preparations for Meiotic Prophase Figures.- 4. Preparations for Meiotic Metaphase Figures.- 5. Interpreting the Findings.- 6. Summary.- References.- XII Marginal Notes on the Handling of Tissue Culture Cells for Biochemical Analysis.- 1. Introduction.- 2. Selected General Notes.- 3. Discussion of Some Procedures.- 4. Presentation of Results.- References.mehr