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Techniques in Endocrine Research

E-BookPDFDRM AdobeE-Book
336 Seiten
Englisch
Elsevier Science & Techn.erschienen am22.10.2013
Techniques in Endocrine Research covers the proceedings of the NATO Advanced Study Institute held at Stratford-upon-Avon, England, under the Auspices of the University of Birmingham. The book focuses on the techniques in endocrinology, as well as x-ray crystallography, electron microscopy, and autoradiography.
The selection first offers information on x-ray crystallography in hormone research and the separation and significance of hormone containing subcellular particles, including formation of granules, specificity of granule storing capacity, chromaffin cell granules, and adrenergic nerve granules. The book also takes a look at the possibilities for the use of antibody staining techniques in electron microscopy and in the study of neurosecretion.
The publication examines problems in the ultrastructure of endocrine glands, with special reference to the mammalian pituitary, autoradiography in endocrine research, and chromatographic methods of separations. The text also ponders on the state, transport, and regulation of hormones in blood; immunochemical methods in hormone research; and interpretation of biochemical techniques. Discussions focus on the state of insulin in human blood, state of pituitary hormones in blood, and transport of thyroxin in blood.
The selection is a vital source of information for readers interested in the techniques in endocrinology.mehr

Produkt

KlappentextTechniques in Endocrine Research covers the proceedings of the NATO Advanced Study Institute held at Stratford-upon-Avon, England, under the Auspices of the University of Birmingham. The book focuses on the techniques in endocrinology, as well as x-ray crystallography, electron microscopy, and autoradiography.
The selection first offers information on x-ray crystallography in hormone research and the separation and significance of hormone containing subcellular particles, including formation of granules, specificity of granule storing capacity, chromaffin cell granules, and adrenergic nerve granules. The book also takes a look at the possibilities for the use of antibody staining techniques in electron microscopy and in the study of neurosecretion.
The publication examines problems in the ultrastructure of endocrine glands, with special reference to the mammalian pituitary, autoradiography in endocrine research, and chromatographic methods of separations. The text also ponders on the state, transport, and regulation of hormones in blood; immunochemical methods in hormone research; and interpretation of biochemical techniques. Discussions focus on the state of insulin in human blood, state of pituitary hormones in blood, and transport of thyroxin in blood.
The selection is a vital source of information for readers interested in the techniques in endocrinology.
Details
Weitere ISBN/GTIN9781483270876
ProduktartE-Book
EinbandartE-Book
FormatPDF
Format HinweisDRM Adobe
Erscheinungsjahr2013
Erscheinungsdatum22.10.2013
Seiten336 Seiten
SpracheEnglisch
Artikel-Nr.3137128
Rubriken
Genre9200

Inhalt/Kritik

Inhaltsverzeichnis
1;Front Cover;1
2;Techniques in Endocrine Research;4
3;Copyright Page;5
4;Table of Contents;16
5;LIST OF PARTICIPANTS;6
6;FOREWORD;10
7;INTRODUCTION;12
8;CHAPTER 1. INAUGURAL LECTURE ON PRESENT PERSPECTIVES IN ENDOCRINOLOGY;18
9;CHAPTER 2. X - RAY CRYSTALLOGRAPHY IN HORMONE RESEARCH;24
9.1;REFERENCES;39
10;CHAPTER 3. THE SEPARATION AND SIGNIFICANCE OF HORMONE-CONTAINING SUBCELLULAR PARTICLES;40
10.1;I. Introduction;40
10.2;II. Formation of granules;41
10.3;III. Demonstration, preparation and isolation of granules;42
10.4;IV· Release;44
10.5;V. Transport;45
10.6;VI. Specificity of granule storing capacity;45
10.7;VII. Acetylcholine;46
10.8;VIII. 5-Hydroxytyramine (5-HT);46
10.9;IX. Histamine;47
10.10;X. Peptide-hormone granules;47
10.11;XI . Other peptide-containing granules;49
10.12;XII. Chromaffin cell granules;49
10.13;XIII. Adrenergic nerve granules;51
10.14;XIV. Conclusions;55
10.15;REFERENCES;55
11;CHAPTER 4. POSSIBILITIES FOR THE USE OF ANTIBODY STAINING TECHNIQUES IN ELECTRON MICROSCOPY;60
11.1;REFERENCES;73
12;CHAPTER 5. TECHNIQUES IN THE STUDY OF NEUROSECRETION;74
12.1;Introduction;74
12.2;Histological methods;74
12.3;Histochemical methods;75
12.4;Electron microscopy;76
12.5;The electrical stimulation of neurosecretory fibres;78
12.6;Discussion;80
12.7;REFERENCES;81
13;CHAPTER 6. SOME PROBLEMS IN THE ULTRASTRUCTURE OF ENDOCRINE GLANDS, WITH SPECIAL REFERENCE TO THE MAMMALIAN PITUITARY;84
13.1;General problems;84
13.2;The pars distalis;85
13.3;Conclusion;89
13.4;REFERENCES;89
14;CHAPTER 7. AUTORADIOGRAPHY IN ENDOCRINE RESEARCH;90
14.1;I. Introduction;90
14.2;II. Hypothalamic control of hypophysial activity using 35S-labelled amino acids;92
14.3;Conclusion;101
14.4;REFERENCES;102
14.5;ACKNOWLEDGMENT;102
15;CHAPTER 8. CHROMATOGRAPHIC METHODS OF SEPARATION;104
15.1;I. Introduction;104
15.2;II. Adsorption chromatography;105
15.3;III. Ion exchange chromatography;109
15.4;IV. Column partition chromatography;112
15.5;REFERENCES;117
15.6;ACKNOWLEDGMENTS;121
16;CHAPTER 9. STATE, TRANSPORT AND REGULATION OF HORMONES IN BLOOD;122
16.1;I. Introduction;122
16.2;II. State of insulin in human blood;127
16.3;III. State of pituitary hormones in blood;135
16.4;IV. Transport of steroid hormones in human blood;139
16.5;V. Transport of thyroxine in blood;142
16.6;VI. State of adrenaline and nor-adrenaline in blood;144
16.7;REFERENCES;145
17;CHAPTER 10. IMMUNOCHEMICAL METHODS IN HORMON ERESEARCH;150
17.1;REFERENCES;159
18;CHAPTER 11. INTERPRETATION OF BIOCHEMICAL TECHNIQUES;160
18.1;Purpose;160
18.2;Interpretation;161
18.3;Further comment;163
19;CHAPTER 12. THE EXPERIMENTAL ANIMAL IN RESEARCH;166
19.1;I. Why animals are used;166
19.2;II. What is expected of the animal;167
19.3;III. How to produce the required animals in quantity;171
19.4;IV. Conclusion;175
19.5;REFERENCES;175
20;CHAPTER 13. THE PITUITARY COMPLEX;178
20.1;Introduction;178
20.2;The pituitary complex;179
20.3;Homologies;179
20.4;Terminology;181
20.5;Morphology;182
20.6;REFERENCES;201
20.7;Acknowledgments;202
21;CHAPTER 14. HORMONES AND GENE ACTION;204
21.1;I. Introduction;204
21.2;II. Interaction of ecdysone with the chromosomes;204
21.3;III. Genes and protein synthesis;206
21.4;IV. Stimulation of enzyme synthesis by ecdysone;207
21.5;V. Mammalian hormones which stimulate protein synthesis;208
21.6;REFERENCES;211
22;CHAPTER 15. TRANSPLANTAION OF ENDOCRINE ORGANS ;212
22.1;Conclusion;217
22.2;REFERENCES;217
23;CHAPTER 16. PRESERVATION AND STORAGE OF ENDOINETISSUE IN A VIABLE STATE;218
23.1;I. Essentials of technique;219
23.2;II. Thyroid tissue;219
23.3;III. Adrenal gland;222
23.4;IV. Testis;223
23.5;V. Ovary;226
23.6;REFERENCES;229
24;CHAPTER 17. PURIFIED PROTEIN HORMONES IN HUMAN ENDOCRINOLOGY;230
24.1;I. Introduction;230
24.2;II. Human follicle stimulating hormone;231
24.3;III. Human growth hormone;240
24.4;III. Human growth hormone;240
24.5;IV. Immunological assay of protein hormones;241
24.6;Conclusion;244
24.7;REFERENCES;246
25;CHAPTER 18. INERDISCIPLINARY APPROACHES TO THE ENDONOLOGY OF REPRODUCTION;248
25.1;The mechanism of implantation;251
25.2;Decidualization;252
25.3;The action of histamine and antihistamine;253
25.4;Induction of decidualization by systemic histamine;254
25.5;The oestrogen surge;256
25.6;REFERENCES;259
26;CHAPTER 19. THE EXPERIMENTAL CONTROL OF OVUM IMPLANTATION;262
26.1;I. Introduction;262
26.2;II. Study of experimental control of the chronology of nidation: influence exerted by the ovary;264
26.3;III. Experimental control of nidation via the hypophysis and central nervous system;267
26.4;IV. Analysis of the experimental control of the uterus and ovum;271
26.5;V. Conclusion;275
26.6;REFERENCES;275
27;CHAPTER 20. HORMONES IN DEVELOPMENT;278
27.1;I. Introduction;278
27.2;II. The function of the hypophysis in the embryo;278
27.3;III. Research on the function of the genital glands in the embryo;289
27.4;IV. Effects of steroid hormones;296
27.5;V. Extension of these researches to the Reptilia;300
27.6;VI. Teratogenic effects of hormones in embryos;303
27.7;REFERENCES;303
28;CHAPTER 21. TRENDS IN THE METHODOLOGY OF ENDOCRINOLOGICAL TECHNIQUES;306
28.1;I. The historical background;306
28.2;II. The concept of levels;310
28.3;III. Variations between species;313
28.4;REFERENCES;319
29;AUTHOR INDEX;320
30;SUBJECT INDEX;331mehr