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An Introduction to Molecular Biotechnology

E-BookEPUB2 - DRM Adobe / EPUBE-Book
520 Seiten
Englisch
Wiley-VCHerschienen am03.12.20203. vollständig überarbeitete Auflage
Completely updated in line with the rapid progress made in the field, this new edition of the highly-praised textbook addresses powerful new methods and concepts in biotechnology, such as genome editing, reprogrammed stem cells, and personalized medicine.
An introduction to the fundamentals in molecular and cell biology is followed by a description of standard techniques, including purification and analysis of biomolecules, cloning techniques, gene expression systems, genome editing methods, labeling of proteins and in situ-techniques, standard and high resolution microscopy. The third part focuses on key areas in research and application, ranging from functional genomics, proteomics and bioinformatics to drug targeting, recombinant antibodies and systems biology. The final part looks at the biotechnology industry, explaining intellectual property issues, legal frameworks for pharmaceutical products and the interplay between start-up and larger companies. The contents are beautifully illustrated throughout, with hundreds of full color diagrams and photographs.
Provides students and professionals in life sciences, pharmacy and biochemistry with everything they need to know about molecular biotechnology.


Michael Wink studied biology and chemistry in Bonn and was awarded his doctorate from TU Braunschweig in 1980. After gaining his lecturing qualification in 1984/1985, he was awarded a Heisenberg grant by the German Research Council to work at the Max Planck Institute for Breeding Research in Cologne and from then at the Gene Center of Ludwig-Maximilians University in Munich. Following a chair for Pharmaceutical Biology at Mainz University in 1988, he accepted the post of Professor for Pharmaceutical Biology at the University of Heidelberg one year later. His areas of interest include pharmaceutical research, molecular biotechnology, and medicinal plants, as well as research into natural products and evolution.
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Verfügbare Formate
BuchKartoniert, Paperback
EUR97,90
E-BookPDF2 - DRM Adobe / Adobe Ebook ReaderE-Book
EUR87,99
E-BookEPUB2 - DRM Adobe / EPUBE-Book
EUR87,99

Produkt

KlappentextCompletely updated in line with the rapid progress made in the field, this new edition of the highly-praised textbook addresses powerful new methods and concepts in biotechnology, such as genome editing, reprogrammed stem cells, and personalized medicine.
An introduction to the fundamentals in molecular and cell biology is followed by a description of standard techniques, including purification and analysis of biomolecules, cloning techniques, gene expression systems, genome editing methods, labeling of proteins and in situ-techniques, standard and high resolution microscopy. The third part focuses on key areas in research and application, ranging from functional genomics, proteomics and bioinformatics to drug targeting, recombinant antibodies and systems biology. The final part looks at the biotechnology industry, explaining intellectual property issues, legal frameworks for pharmaceutical products and the interplay between start-up and larger companies. The contents are beautifully illustrated throughout, with hundreds of full color diagrams and photographs.
Provides students and professionals in life sciences, pharmacy and biochemistry with everything they need to know about molecular biotechnology.


Michael Wink studied biology and chemistry in Bonn and was awarded his doctorate from TU Braunschweig in 1980. After gaining his lecturing qualification in 1984/1985, he was awarded a Heisenberg grant by the German Research Council to work at the Max Planck Institute for Breeding Research in Cologne and from then at the Gene Center of Ludwig-Maximilians University in Munich. Following a chair for Pharmaceutical Biology at Mainz University in 1988, he accepted the post of Professor for Pharmaceutical Biology at the University of Heidelberg one year later. His areas of interest include pharmaceutical research, molecular biotechnology, and medicinal plants, as well as research into natural products and evolution.
Details
Weitere ISBN/GTIN9783527812882
ProduktartE-Book
EinbandartE-Book
FormatEPUB
Format Hinweis2 - DRM Adobe / EPUB
FormatFormat mit automatischem Seitenumbruch (reflowable)
Verlag
Erscheinungsjahr2020
Erscheinungsdatum03.12.2020
Auflage3. vollständig überarbeitete Auflage
Seiten520 Seiten
SpracheEnglisch
Dateigrösse29155 Kbytes
Artikel-Nr.5503716
Rubriken
Genre9201

Inhalt/Kritik

Inhaltsverzeichnis
FUNDAMENTALS OF CELLULAR AND MOLECULAR BIOLOGY
The Cell as the Basic Unit of Life
Structure and Function of Cellular Macromolecules
Structure and Functions of a Cell
Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins)
Distributing Proteins in the Cell (Protein Sorting)
Evolution and Diversity of Organisms

STANDARD METHODS IN MOLECULAR BIOTECHNOLOGY
Isolation and Purification of Proteins
Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry
Isolation of DNA and RNA
Chromatography and Electrophoresis of Nucleic Acids
Hybridization of Nucleic Acids
Use of Enzymes in the Modification of Nucleic Acids
Polymerase Chain Reaction
DNA Sequencing
Genome Editing
Cloning Procedures
Expression of Recombinant Proteins
Omics technologies
Patch Clamp Method
Cell Cycle Analysis
Microscopic Techniques
Laser Applications

KEY TOPICS
Genomics and Functional Genomics
Bioinformatics
Cellular Systems Biology
Protein-Protein and Protein-DNA Interaction
Drug Research
Drug Targeting and Prodrugs
Molecular Diagnostics and Personalized Medicine
Recombinant Antibodies and Phage Display
Transgenic and Gene-Targeted Mice
Plant Biotechnology
Industrial Biocatalysis

BIOTECHNOLOGY IN INDUSTRY
The Biotech Industry, Markets, and Opportunities
Patents in the Molecular Biotechnology Industry: Legal and Ethical Issues
Drug Approval in the European Union and United States
Founding a Biotech Company

APPENDIX
Further Reading
Abbreviations
Subject Index
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Leseprobe

Abbreviations
1ââ« =0.1ânm aa-tRNA aminoacyl-tRNA AAV adeno-associated virus ABC ATP-binding cassette Acetyl CoA acetyl coenzyme A AcNPV Autographa californica nuclear polyhedrosis virus ACRS amplification-created restriction sites ACTH adrenocorticotropic hormone ADA adenosine deaminase ADEPT antibody-directed enzyme prodrug therapy ADME-T absorption, distribution, metabolism, excretion, and toxicity ADP adenosine diphosphate ADRs adverse drug reactions AEC aminoethylcysteine AFLP amplified fragment length polymorphism AFM atomic force microscope AIDS acquired immunodeficiency syndrome ALS amyotrophic lateral sclerosis AMP adenosine monophosphate AMPA α-amino-3-hydroxyl-5-methyl-4-isoxazol-propionate Ampr ampicillin resistance gene AMV avian myeloblastosis virus ANN artificial neural network AO acridine orange AOX1 alcohol oxidase 1 APC anaphase-promoting complex ApoB100 apolipoprotein B100 ApoE apolipoprotein E APP amyloid precursor protein ARMS amplification refractory mutation system ARS autonomously replicating sequence ATP adenosine triphosphate att attachment site BAC bacterial artificial chromosome bcl2 B-cell leukemia lymphoma 2 (protein protecting against apoptosis) BfArM German Bundesinstitut für Arzneimittel und Medizinprodukte β-Gal β-galactosidase BHK-21 baby hamster kidney cells BLA biologics licence application BLAST Basic Local Alignment Search Tool BMP bone morphogenetic proteins bp base pairs BrdU bromodeoxyuridine CA correspondence analysis CAD coronary artery disease CaM-Kinase Ca2+/calmodulin-dependent protein kinase cAMP cyclic AMP cap AAV gene mediating encapsulation CARS coherent anti-Raman scattering CAT Committee for Advanced Therapies CBER Center for Biologics Evaluation and Research CC chromatin remodeling complex CCD charge-coupled device CDER Center for Drug Evaluation and Research CDK cyclin-dependent kinase cDNA copy DNA CDR complementarity-determining region CDRH Center for Devices and Radiological Health CEO chief executive officer CFP cyan fluorescent protein CFTR cystic fibrosis transmembrane regulator CGAP Cancer Genome Anatomy Project CGH comparative genome hybridization CHMP Committee for Medicinal Products for Human Use CHO Chinese hamster ovary CIP calf intestinal phosphatase CML chronic myeloid leukemia CMN Corynebacterium-Mycobacterium-Nocardia group CaMV cauliflower mosaic virus CMV cytomegalovirus CNS central nervous system COMP Committee for Orphan Medicinal Products COS-1 simian cell line, CV-1, transformed by origin-defective mutant of SV40 cpDNA chloroplast DNA CPMV cowpea mosaic virus cPPT-sequence central polypurine tract - regulatory element in lentiviral vectors that facilitates double strand synthesis and the nuclear import of the pre-integration complex CSF colony-stimulating factor CSO contract service organization CTAB cetyltrimethylammonium bromide CVM Center for Veterinary Medicine CVMP Committee for Medicinal Products for Veterinary Use 2D two-dimensional Da Dalton DAG diacylglycerol DAPI 4,6-diamidino-2-phenylindole dATP deoxyadenosine triphosphate DBD DNA-binding domain DAC divide-and-conquer strategy DD differential display DDBJ DNA Data Bank of Japan ddNTP dideoxynucleotide triphosphate DEAE diethylaminoethyl dHPLC denaturing HPLC DIC differential interference contrast DIP Database of Interacting Proteins DNA deoxyribonucleic acid DNAse deoxyribonuclease dNTP deoxynucleoside triphosphate Dox doxycycline ds diabodies disulfide-stabilized diabodies dsDNA double-stranded DNA dsFv-fragment disulfide-stabilized Fv fragment dsRNA double-stranded RNA DtxR diphtheria toxin repressor Ebola-Z envelope protein of the Ebola-Zaire virus, which has a high affinity to lung epithelial cells EC50 effective concentration, the dose or concentration that produces a 50% effect in the test population within a specified time ECD electron capture dissociation EDTA ethylenediaminetetraacetic acid ee enantiomeric excess EF2 elongation factor 2 EF-Tu elongation factor Tu EGF epidermal growth factor EGFP enhanced green fluorescent protein EGTA ethylene glycol bis(2-aminoethyl)tetraacetic acid EIAV equine infectious anemia virus ELISA enzyme-linked immunosorbent assay EM electron microscope EMA European Medicines Agency EMBL European Molecular Biology Laboratory EMCV encephalomyocarditis...
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