Hugendubel.info - Die B2B Online-Buchhandlung 

Merkliste
Die Merkliste ist leer.
Bitte warten - die Druckansicht der Seite wird vorbereitet.
Der Druckdialog öffnet sich, sobald die Seite vollständig geladen wurde.
Sollte die Druckvorschau unvollständig sein, bitte schliessen und "Erneut drucken" wählen.

Bacterial Regulatory RNA

E-BookPDF1 - PDF WatermarkE-Book
418 Seiten
Englisch
Springer New Yorkerschienen am14.02.20242nd ed. 2024
This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Bacterial Regulatory RNA: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.mehr
Verfügbare Formate
BuchGebunden
EUR213,99
E-BookPDF1 - PDF WatermarkE-Book
EUR209,00

Produkt

KlappentextThis second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Bacterial Regulatory RNA: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Details
Weitere ISBN/GTIN9781071635650
ProduktartE-Book
EinbandartE-Book
FormatPDF
Format Hinweis1 - PDF Watermark
FormatE107
Erscheinungsjahr2024
Erscheinungsdatum14.02.2024
Auflage2nd ed. 2024
ReiheMethods in Molecular Biology
Reihen-Nr.2741
Seiten418 Seiten
SpracheEnglisch
IllustrationenXII, 418 p. 97 illus., 77 illus. in color.
Artikel-Nr.13438093
Rubriken
Genre9200

Inhalt/Kritik

Inhaltsverzeichnis
RNA extraction from Gram-positive bacteria membrane vesicles using a polymer-based precipitation method.- Extraction and purification of outer membrane vesicles and their associated RNAs.- Analysis of phage regulatory RNAs: sequencing library construction from the fraction of small prokaryotic RNAs less than 50 nucleotides in length.- Discovering Novel Bacterial Small RNA by RNA-seq Analysis Toolkit ANNOgesic.- Ribosome profiling methods adapted to the study of RNA-dependent translation regulation in Staphylococcus aureus.- CRISPR interference-based functional small RNA genomics.- Investigation of sRNA-mRNA interactions in Bacillus subtilis in vivo.- In vitro methods for the investigation of sRNA-mRNA interactions in Bacillus subtilis.- RNA double helix hybridization measured by fluorescence correlation spectroscopy.- New perspectives on cross talks between bacterial regulatory RNAs from outer membrane vesicles and eukaryotic cells.- Experimental validation of RNA-RNA interactions by Electrophoretic Mobility Shift Assay.- Dynamics and function of sRNA/mRNAs under the scrutiny of computational simulation methods.-Analysis of sRNAs and their mRNA targets in Sinorhizobium meliloti: focus on half-live determinationEvaluation of 5'-end phosphorylation for small RNA stability and target regulation in vivo.- In-gel cyanoethylation for pseudouridines mass spectrometry detection of bacterial regulatory RNA.- Directed screening for sRNA targets in E. coli using a plasmid library.- Defining Bacterial RNA-RNA Interactomes Using CLASH.- Global identification of RNA-binding proteins in bacteria.- An integrated affinity chromatography-based approach to unravel the sRNA interactome in nitrogen-fixing rhizobia.- sRNA structural modeling based on NMR data.- Circular and linear dichroism for the analysis of small noncoding RNA properties.mehr