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Vectorology for Optogenetics and Chemogenetics

BuchKartoniert, Paperback
333 Seiten
Englisch
Springererschienen am07.02.20241st ed. 2023
The chapters in this book are organized into three parts detailing (1) viral vectors, (2) vector modifications and applications, and (3) practical advice for the delivery of viral vectors, verification of injection accuracy, and monitoring of transgene expression.mehr
Verfügbare Formate
BuchGebunden
EUR213,99
BuchKartoniert, Paperback
EUR139,09

Produkt

KlappentextThe chapters in this book are organized into three parts detailing (1) viral vectors, (2) vector modifications and applications, and (3) practical advice for the delivery of viral vectors, verification of injection accuracy, and monitoring of transgene expression.
Details
ISBN/GTIN978-1-0716-2920-8
ProduktartBuch
EinbandartKartoniert, Paperback
Verlag
Erscheinungsjahr2024
Erscheinungsdatum07.02.2024
Auflage1st ed. 2023
ReiheNeuromethods
Seiten333 Seiten
SpracheEnglisch
IllustrationenXIII, 333 p. 71 illus., 64 illus. in color.
Artikel-Nr.55854633
Rubriken
GenreMedizin

Inhalt/Kritik

Inhaltsverzeichnis
Production, testing and verification of Lentivirus for regional targeting in the old-world monkey brain.- HiRet/NeuRet vectors: Lentiviral system for highly efficient gene transfer through retrograde axonal transport.-Generation of High-Titer Defective HSV-1 Amplicon Vectors.- Generation and Application of Engineered Rabies Viral Vectors for Neural Circuit Research.- Quality control for Adeno-associated viral vector production.- Vector tropism.-Viruses for systemic delivery.- Transcriptomic Definition of Neuron Types.- Enhancers for selective targeting.- Pathway-selective reversible perturbations using a double-infection technique in the macaque brain.- Pathway-Specific Chemogenetic Manipulation by Applying Ligand to Axonally-Expressed DREADDs.- Convection-enhanced delivery of viral vectors.- Multi-channel microinjector arrays for efficient viral vector delivery into rhesus monkey brain.- Methods to verify expression and function of DREADDs using PET.- Reporter selection and post-mortem methods to verify transgene expression.- Considerations for the use of viral vectors in NHP experiments.mehr