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Animal Cell Culture: Principles and Practice

BuchGebunden
327 Seiten
Englisch
Springererschienen am02.02.20231st ed. 2023
This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques.mehr
Verfügbare Formate
BuchGebunden
EUR139,09
BuchKartoniert, Paperback
EUR96,29
E-BookPDF1 - PDF WatermarkE-Book
EUR96,29

Produkt

KlappentextThis introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques.
Details
ISBN/GTIN978-3-031-19484-9
ProduktartBuch
EinbandartGebunden
Verlag
Erscheinungsjahr2023
Erscheinungsdatum02.02.2023
Auflage1st ed. 2023
ReiheTechniques in Life Science and Biomedicine for the Non-Expert
Seiten327 Seiten
SpracheEnglisch
IllustrationenXVI, 327 p. 91 illus., 85 illus. in color.
Artikel-Nr.51086002

Inhalt/Kritik

Inhaltsverzeichnis
1. Overview to animal cell culture.- 2. Cell culture laboratory.- 3. Good lab practices and bio-safety measures.- 4. Managing sterility in the lab.- 5. Media and buffer preparation for cell culture.- 6. Properties of cultured cells and selection of culture media.- 7. Selection and maintenance of cultured cells.- 8. Inoculation and passaging of adherent cells.- 9. Counting of cells.- 10. Cryopreservation of cell lines.- 11. Resuscitation of frozen cell line.- 12. Isolation and culturing  of cells from different tissues.- 13. Differentiation, transformation and immortalization of cells.- 14. Co-culture techniques.- 15. 3d cell culture techniques.- 16. Stem cell culture techniques.- 17. Identification and removal of biological contamination in the media and cell suspensions.- 18. Analysis of cell growth kinetics in suspension and adherent types cell line.-  19. Applications of animal cell culture based assays.- 20. Ethical issues in animal cell culture.- 21. Common troubleshooting methods in cell culture techniques.mehr

Schlagworte

Autor

Dr. Shalini Mani is Associate Professor in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Manisha Singh is Assistant Professor and Lecturer in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Anil Kumar is Director of Education and Professor at RLB Central Agricultural University, Jhansi, India.
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Mani, Shalini