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Resin Microscopy and On-Section Immunocytochemistry

BuchKartoniert, Paperback
273 Seiten
Englisch
Springererschienen am23.08.20142. Aufl.
Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation.mehr
Verfügbare Formate
BuchKartoniert, Paperback
EUR160,49
BuchGebunden
EUR213,99
E-BookPDF1 - PDF WatermarkE-Book
EUR149,79

Produkt

KlappentextSince antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation.
Zusammenfassung
Ready-to-go protocols

Explains all steps from fixation to labelling and detection

Helps in finding the resin of choice for your needs
Details
ISBN/GTIN978-3-642-47731-7
ProduktartBuch
EinbandartKartoniert, Paperback
Verlag
Erscheinungsjahr2014
Erscheinungsdatum23.08.2014
Auflage2. Aufl.
ReiheSpringer Lab Manuals
Seiten273 Seiten
SpracheEnglisch
Gewicht580 g
IllustrationenXIX, 273 p.
Artikel-Nr.32592773

Inhalt/Kritik

Inhaltsverzeichnis
I: Resin Embedding.- 1 The Strategic Approach.- 2 The Resins.- 3 Resin Embedding Protocols for Chemically Fixed Tissue.- 4 Cryotechniques.- 5 Methods for Resin Polymerisation.- 6 Handling Resin Blocks.- II: On-Section Immunolabelling.- 7 Strategies in Immunolabelling.- 8 General Considerations.- 9 Immunolabelling Protocols for Resin Sections.- 10 Resin Embedding and Immunolabelling.- Appendix I Examples of Typical Resin Embedding Regimes for Immunocytochemistry.- I.1 Solid Tissue, Pellets and Agar Blocks - Immersion Fixation.- I.2 Solid Tissue - Light Perfusion Fixation.- I.3 Solid Tissue - Very Light Perfusion Fixation.- I.4 Cryosubstitution.- I.5 Project Planner.- Appendix II List of Suppliers.- EM General.- EM Apparatus.- Electron Microscopes.- Flow Cytometry.- References.mehr
Kritik
From the reviews of the second edition:



"This comprehensive and valuable book is a must-have for the immunohistochemical laboratory. It extensively covers the field of onsection immunocytochemistry, including epoxy and acrylic resins, a variety of embedding and immunolabelling protocols ... . It is the only book I know covering LR-White and -Gold, the different Lowicryls, and Unicryl in this comprehensive manner. ... it is a very helpful book and because of its extensive theoretical and practical reference a standard for the beginner as well as those already familiar with immunocytochemistry." (Jens Krieger, Microscopy and Analysis, September, 2002)mehr