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Fingerprinting Methods Based on Arbitrarily Primed PCR

BuchKartoniert, Paperback
441 Seiten
Englisch
Springererschienen am23.08.2014Softcover reprint of the original 1st ed. 1997
DNA and RNA fingerprinting based on arbitrarily primed PCR provides the most powerful tool for the study of genes.The basic techniques are described in detailed protocols including each step from template preparation to fingerprint visualization.mehr
Verfügbare Formate
BuchKartoniert, Paperback
EUR53,49
E-BookPDF1 - PDF WatermarkE-Book
EUR53,49

Produkt

KlappentextDNA and RNA fingerprinting based on arbitrarily primed PCR provides the most powerful tool for the study of genes.The basic techniques are described in detailed protocols including each step from template preparation to fingerprint visualization.
Details
ISBN/GTIN978-3-642-47812-3
ProduktartBuch
EinbandartKartoniert, Paperback
Verlag
Erscheinungsjahr2014
Erscheinungsdatum23.08.2014
AuflageSoftcover reprint of the original 1st ed. 1997
ReiheSpringer Lab Manuals
Seiten441 Seiten
SpracheEnglisch
Gewicht979 g
IllustrationenXVI, 441 p.
Artikel-Nr.32592776

Inhalt/Kritik

Inhaltsverzeichnis
Overview.- I DNA Extraction from Mammals.- II Insect DNA Extraction Protocol.- III Rapid DNA Extraction from Plants.- IV Preparation of Fungal Genomic DNA for PCR and RAPD Analysis.- V Extraction of Histoplasma capsulatum DNA for PCR.- VI DNA Extraction from Bacterial Cultures.- VII Arbitrarily Primed PCR and RAPDs.- VIII Random Amplified Polymorphic DNA Assay.- IX DNA Amplification Fingerprinting.- X Fluorescent Detection and Analysis of RAPD Amplicons Using the ABI PRISM DNA Sequencers.- XI Optimization of RAPD Fingerprinting.- XII Fingerprint Tailoring.- XIII Resolving DNA Amplification Products Using Polyacrylamide Gel Electrophoresis and Silver Staining.- XIV Denaturing Gradient Gel Electrophoresis for Enhanced Detection of DNA Polymorphisms.- XV Modified Temperature Sweep Gel Electrophoresis for the Separation of Arbitrarily Amplified DNA Fragments.- XVI High Throughput Scoring of RAPD Fragments Through the Use of Dot-Blot Hybridization.- XVII Recovering Amplified DNA from Silver Stained Gels.- XVIII Cloning of RAPD Markers.- XIX Sequencing of RAPD Markers.- XX Analysis of Tumor-Specific Genetic Alterations by Arbitrarily Primed PCR.- XXI Construction of Linkage Maps with RAPD Markers.- XXII Pseudo-Testcross Mapping Strategy Using RAPD Markers.- XXIII Estimating Nucleotide Divergence with RAPD Data.- XXIV RAPD and PAUP Analysis for Microbial Screening Programs.- XXV Production of Specific Probes for Microorganisms.- Overview.- XXVI Differential Display of Expressed mRNAs.- XXVII RNA Arbitrarily Primed PCR.- XXVIII Nonradioactive Differential Display of Messenger RNA.- XXIX Fluorescent Differential Display: A Fast and Safe Way for Reliable Differential Display Analysis.- XXX Slot Blot Hybridization Screening.- XXXI How To Find and Clone the Appropriate cDNA Fragments Generated in Differential mRNA Display by Using Northern Blot for cDNA Capture.- XXXII Verification of Differential Display Results by RNase Protection.- XXXIII Direct Automated Sequencing of DDRT-PCR Fragments.- XXXIV Ligation Linked PCR and Direct Sequencing of Differential Display Products.- XXXV Differential Display PCR Fragments as Probes for cDNA Cloning.- XXXVI Targeted RNA Fingerprinting.- XXXVII Generation of Tumor-Specific DEST Catalogue.- XXXVIII Analysis of Gene Expression in the Preimplantation Mouse Embryo Using mRNA Differential Display.- XXXIX Rapid Identification of Differentially Expressed Genes in Trypanosomes.mehr
Kritik
"The only primer you'll ever need... great care has been taken in putting this book together, and the results are very impressive. Although the work of many authors, the contributions form a coherent and well-organized whole." Trends in Genetics
"...buy this excellent book." Cellular and Molecular Biologymehr