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Nucleic Acid Aptamers

E-BookPDF1 - PDF WatermarkE-Book
Englisch
Springer USerschienen am26.09.20222nd ed. 2023
Verfügbare Formate
BuchGebunden
EUR160,49
E-BookPDF1 - PDF WatermarkE-Book
EUR106,99

Produkt

Details
Weitere ISBN/GTIN9781071626955
ProduktartE-Book
EinbandartE-Book
FormatPDF
Format Hinweis1 - PDF Watermark
FormatE107
Erscheinungsjahr2022
Erscheinungsdatum26.09.2022
Auflage2nd ed. 2023
ReiheMethods in Molecular Biology
Reihen-Nr.2570
SpracheEnglisch
IllustrationenXI, 282 p. 78 illus., 66 illus. in color.
Artikel-Nr.9562383
Rubriken
Genre9200

Inhalt/Kritik

Inhaltsverzeichnis
Automated ssDNA SELEX Using Histidine Tagged Target Proteins.- Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection.- Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX.- Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction.- RNA Capture-SELEX on Streptavidin Magnetic Beads.- The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE.- Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX.- Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry.- Determination of Aptamer Structure Using Circular Dichroism Spectroscopy.- Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis.- Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance.- Probing of Fluorogenic RNA Aptamers via SupramolecularFörster Resonance Energy Transfer with Universal Fluorescent Nucleobase Analog.- Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT.- Aptamer-Field-Effect Transistors for Small-Molecule Sensing in Complex Environments.- Aptasensor for Impedimetric Detection of Lysozyme.- Biophysical Analysis of Small Molecule Binding to Viral RNA Structures.- Designing and Expressing Circular RNA Aptamers to Regulate Mammalian Cell Biology.- Enzyme-Linked OligoNucleotide Assay (ELONA).- High-Throughput Development and Optimization of RNA-Based Fluorogenic Biosensors of Small Molecules Using Droplet-Based Microfluidics.- Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical Analysis.mehr